Array ( [0] => {{short description|Oldest cultured human cell line (1951)}} [1] => {{Other uses|Hela (disambiguation)}} [2] => {{Use American English|date=December 2022}} [3] => {{Use mdy dates|date=December 2022}} [4] => [[File:HeLa-IV.jpg|thumb| Scanning electron micrograph of an [[Apoptosis|apoptotic]] HeLa cell. Zeiss Merlin HR-SEM.]] [5] => [[File:HeLa-I.jpg|thumb|Multiphoton fluorescence image of cultured HeLa cells with a fluorescent protein targeted to the [[Golgi apparatus]] (orange), microtubules (green) and counterstained for DNA (cyan). Nikon RTS2000MP custom laser scanning microscope.]] [6] => [[File:HeLa cells stained with antibody to actin (green) , vimentin (red) and DNA (blue).jpg|thumb|[[Immunofluorescence]] image of HeLa cells grown in tissue culture and stained with antibody to [[actin]] in green, [[vimentin]] in red and DNA in blue]] [7] => [[File:HeLa-Tubulin-HSP60-Fibrillarin-DNA.jpg|thumb|[[Immunofluorescence]] of HeLa cells showing [[microtubules]] in green, [[mitochondria]] in yellow, [[nucleoli]] in red and nuclear DNA in purple]] [8] => '''HeLa''' ({{IPAc-en|ˈ|h|iː|l|ɑː}}) is an [[Immortalised cell line|immortalized cell line]] used in scientific research. It is the oldest [[human cell line]] and one of the most commonly used.{{cite journal|vauthors= Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM |title=A novel L1 retrotransposon marker for HeLa cell line identification |journal=BioTechniques |volume=46 |issue=4 |pages=277–284 |year=2009 |pmid=19450234 |pmc=2696096 |doi=10.2144/000113089 }}{{Cite web |last=Morris |first=Rhys Bowen |date=2023-08-02 |title=What were the top 100 cell lines of 2022? |url=https://blog.citeab.com/what-were-the-top-100-cell-lines-of-2022/ |access-date=2023-08-17 |website=CiteAb Blog |language=en}} HeLa cells are durable and prolific, allowing for extensive applications in scientific study.{{cite journal |vauthors=Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI |year=2010 |title=Check your cultures! A list of cross-contaminated or misidentified cell lines |journal=Int. J. Cancer |volume=127 |issue=1 |pages=1–8 |doi=10.1002/ijc.25242 |pmid=20143388 |s2cid=2929020 |doi-access=free}}{{cite web |author=Batts DW |date=2010-05-10 |title=Cancer cells killed Henrietta Lacks – then made her immortal |url=http://hamptonroads.com/2010/05/cancer-cells-killed-henrietta-lacks-then-made-her-immortal |url-status=dead |archive-url=https://web.archive.org/web/20161125100920/http://pilotonline.com/news/local/health/cancer-cells-killed-henrietta-lacks---then-made-her/article_17bd351a-f606-54fb-a499-b6a84cb3a286.html |archive-date=2016-11-25 |access-date=2020-05-08 |publisher=The Virginian-Pilot |pages=1, 12–14}} The line is derived from [[cervical cancer]] cells taken on 8 February 1951,{{cite journal |author1=Scherer, W.F. |author2=Syverton, J.T. |author3=Gey, G.O. |title=Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix |journal=Journal of Experimental Medicine |volume=97 |issue=5 |pages=695–710 |year=1953 |pmid=13052828 |pmc=2136303 |doi=10.1084/jem.97.5.695}} from [[Henrietta Lacks]], a 31-year-old African American mother of five, after whom the line is named. Lacks died of cancer on 4 October 1951.{{Cite web|url=https://pages.jh.edu/jhumag/0400web/01.html|title=Johns Hopkins Magazine -- April 2000|website=pages.jh.edu}} [9] => [10] => The cells from Lacks's cancerous cervical tumor were taken without her knowledge, which was common practice in the United States at the time.{{cite web |url=https://abcnews.go.com/WN/womans-cells-changed-medicine/story?id=9712579 |title=How One Woman's Cells Changed Medicine |access-date=2012-08-19 |author1=Ron Claiborne |author2=Sydney Wright, IV |publisher=[[ABC World News]] |date=2010-01-31}} [[Cell biologist]] [[George Otto Gey]] found that they could be kept alive,{{cite news |title=Henrietta Lacks's cells were priceless, but her family can't afford a hospital |newspaper=The Guardian |location=London |url=http://theguardian.com/world/2010/apr/04/henrietta-lacks-cancer-cells |access-date=18 July 2017|date=2010-04-03 |last1=McKie |first1=Robin}} and developed a [[cell line]]. Previously, cells cultured from other human cells would survive for only a few days, but cells from Lacks's tumor behaved differently. [11] => [12] => ==History== [13] => ===Origin=== [14] => In 1951, a woman named [[Henrietta Lacks]] was admitted to the [[Johns Hopkins Hospital]] with symptoms of irregular vaginal bleeding; she was subsequently treated for cervical cancer.{{Cite journal|last1=Lucey|first1=Brendan P.|last2=Nelson-Rees|first2=Walter A.|last3=Hutchins|first3=Grover M.|date=2009-10-21|title=Henrietta Lacks, HeLa Cells, and Cell Culture Contamination|url=https://www.archivesofpathology.org/doi/10.1043/1543-2165-133.9.1463|journal=Archives of Pathology & Laboratory Medicine|language=EN|volume=133|issue=9|pages=1463–7|doi=10.5858/133.9.1463|pmid=19722756}} Her first treatment was performed by Lawrence Wharton Jr., who at that time collected tissue samples from her cervix without her consent.{{Cite journal|last=Jones|first=Howard W.|date=1997-06-01|title=Record of the first physician to see Henrietta Lacks at the Johns Hopkins Hospital: History of the beginning of the HeLa cell line|url=https://www.sciencedirect.com/science/article/pii/S000293789770379X|journal=American Journal of Obstetrics and Gynecology|language=en|volume=176|issue=6|pages=s227–s228|doi=10.1016/S0002-9378(97)70379-X|pmid=9215212|issn=0002-9378}} Her cervical biopsy supplied samples of tissue for clinical evaluation and research by [[George Otto Gey]], head of the Tissue Culture Laboratory. Gey's lab assistant Mary Kubicek used the roller-tube technique to culture the cells. It was observed that the cells grew robustly, doubling every 20–24 hours, unlike previous specimens, which died out.{{Cite web|last=Butanis|first=Benjamin|title=The Legacy of Henrietta Lacks|url=https://www.hopkinsmedicine.org/henriettalacks/|access-date=2020-05-07|website=www.hopkinsmedicine.org|language=en}} [15] => [16] => The cells were propagated by Gey shortly before Lacks died of her cancer in 1951. This was the first human cell line to prove successful [[in vitro]], which was a scientific achievement with profound future benefit to medical research. Gey freely donated these cells, along with the tools and processes that his lab developed, to any scientist requesting them, simply for the benefit of science. Neither Lacks nor her family gave permission to harvest the cells.Washington, Harriet "Henrietta Lacks: An Unsung Hero", Emerge Magazine, October 1994 The cells were later commercialized, although never patented in their original form. There was no requirement at that time to inform patients or their relatives about such matters, because discarded material or material obtained during surgery, diagnosis, or therapy was the property of the physician or the medical institution.{{cn|date=May 2023}} [17] => [18] => As was customary for Gey's lab assistant, the culture was named after the first two letters of Henrietta Lacks' first and last names, He + La. Before a leak to the public in the 1970s, which revealed her true name, the "HeLa" cell line was mistakenly believed to have been named after a "Helen Lane" or "Helen Larson".{{cite web|url=https://www.smithsonianmag.com/science-nature/henrietta-lacks-immortal-cells-6421299/|title=Cracking the code of the human genome – Henrietta Lacks' 'immortal' cells|last1=Zielinski|first1=Sarah|date=2010-01-02|publisher=Smithsonian|access-date=2017-05-27}} [19] => [20] => When other cell cultures were being invaded by suspected HeLa cells, one research group contacted the Lacks family,{{when|date=August 2023}} seeking DNA samples to help identify contaminating cell lines. The family never understood the purpose of the visit, but they were distressed by their understanding of what the researchers told them.{{citation |title= Descendants of Henrietta Lacks discuss her famous cell line |date= May 2, 2018 |first= Tracie |last= White |work= Stanford Medicine News Center |url= https://med.stanford.edu/news/all-news/2018/05/descendants-of-henrietta-lacks-discuss-her-famous-cell-line.html |access-date= 2021-12-09}} These cells are treated as cancer cells, as they are descended from a biopsy taken from a visible lesion on the cervix as part of Lacks's diagnosis of cancer.{{cite web|url=http://berkeleysciencereview.com/article/good-bad-hela/|title=The Good, the Bad, and the HeLa|last1=del Carpio|first1=Alexandra|date=April 27, 2014|publisher=Berkeley Science Review|access-date=2017-05-27|archive-date=2017-05-23|archive-url=https://web.archive.org/web/20170523133626/http://berkeleysciencereview.com/article/good-bad-hela/|url-status=dead}} [21] => [22] => HeLa cells, like other cell lines, are termed "[[Immortalised cell line|immortal]]" because they can divide an unlimited number of times in a laboratory cell culture plate, as long as fundamental cell survival conditions are met (i.e. being maintained and sustained in a suitable environment). There are many [[Strain (biology)|strains]] of HeLa cells, because they mutate during division in [[cell culture]]s, but all HeLa cells are descended from the same tumor cells removed from Lacks. The total number of HeLa cells that have been propagated in cell culture far exceeds the total number of cells that were in Henrietta Lacks's body.{{cite journal|author=Sharrer T|year=2006|title="HeLa" Herself|url=http://www.the-scientist.com/?articles.view/articleNo/24114/title/-HeLa--Herself/|journal=[[The Scientist (magazine)|The Scientist]]|volume=20|issue=7|page=22}} [23] => [24] => ===Controversy=== [25] => [[File:Henrietta Lacks statue, Bristol, RHS.jpg|thumb|Statue of Henrietta Lacks unveiled October 2021 at [[Royal Fort House]], Bristol]] [26] => Lacks's case is one of many examples of the lack of [[informed consent]] in 20th-century medicine. Communication between tissue donors and doctors was virtually nonexistent{{emdash}}cells were taken without patient consent, and patients were not told what the cells would be used for. Johns Hopkins Hospital, where Lacks received treatment and had her tissue harvested, was the only hospital in the Baltimore area where African American patients could receive free care. The patients who received free care from this segregated sect of the hospital often became research subjects without their knowledge.{{Cite journal|last=Stump|first=Jessica L.|date=2014|title=Henrietta Lacks and The HeLa Cell: Rights of Patients and Responsibilities of Medical Researchers|journal=The History Teacher|volume=48|issue=1|pages=127–180|jstor=43264385|issn=0018-2745}} Lacks's family also had no access to her patient files and had no say in who received HeLa cells or what they would be used for. Additionally, as HeLa cells were popularized and used more frequently throughout the scientific community, Lacks's relatives received no financial benefit and continued to live with limited access to healthcare.{{Cite web|last=Day|first=Jo Ann|title=Upholding the Highest Bioethical Standards {{!}} Johns Hopkins Medicine|url=https://www.hopkinsmedicine.org/henriettalacks/upholding-the-highest-bioethical-standards.html|access-date=2020-04-15|website=www.hopkinsmedicine.org|language=en}} [27] => [28] => This issue of who owns tissue samples taken for research was brought up in the [[Supreme Court of California]] case of ''[[Moore v. Regents of the University of California]]'' in 1990. The court ruled that a person's discarded tissue and cells are not his or her property and can be commercialized. [29] => [30] => Lacks's case influenced the establishment of the [[Common Rule]] in 1981. The Common Rule enforces informed consent by ensuring that doctors inform patients if they plan to use any details of the patient's case in research and give them the choice of disclosing the details or not. Tissues connected to their donors' names are also strictly regulated under this rule, and samples are no longer named using donors' initials, but rather by code numbers. To further resolve the issue of patient privacy, Johns Hopkins established a joint committee with the NIH and several of Lacks's family members to determine who receives access to Henrietta Lacks's genome.{{Cite web|title=HeLa Cell Line Origins, Contamination, Controversy, and Cytogenetics.|url=https://www.hela-transfection.com/|language=en-US|access-date=2020-04-15}} [31] => [32] => In 2021, Henrietta Lacks's estate sued to get past and future payments for the alleged unauthorized and widely known sale of HeLa cells by [[Thermo Fisher Scientific]].{{Cite web|url=https://endpts.com/estate-of-henrietta-lacks-sues-thermo-fisher-over-the-improper-sale-of-her-immortal-cells/|title = Estate of Henrietta Lacks sues Thermo Fisher over the improper sale of her immortal cells}} Lacks's family hired an attorney to seek compensation from upwards of 100 pharmaceutical companies that have used and profited from HeLa cells.{{Cite news|title=Family of Henrietta Lacks hires civil rights attorney to seek funds over famous cells|language=en-US|newspaper=Washington Post|url=https://www.washingtonpost.com/local/henrietta-lacks-cells-family-compensation/2021/07/31/63935928-f16c-11eb-bf80-e3877d9c5f06_story.html|access-date=2021-12-11|issn=0190-8286}} Settlement of the suit with Thermo Fisher Scientific was announced in August 2023, with undisclosed terms.{{cite news |last1=Holpuch |first1=Amanda |title=Family of Henrietta Lacks Settles With Biotech Company That Used Her Cells |url=https://www.nytimes.com/2023/08/01/science/henrietta-lacks-cells-lawsuit-settlement.html |work=The New York Times |date=1 August 2023}} Subsequent to the settlement it was announced that the Lacks family was suing the company [[Ultragenyx]].{{Cite web|title=Ultragenyx Sued by Henrietta Lacks' Family in Second HeLa Cell Line Lawsuit|url=https://www.biospace.com/article/ultragenyx-hela-lawsuit-placeholder/|language=en-US|access-date=2023-08-11}} [33] => [34] => ==Use in research== [35] => HeLa cells were the first human cells to be successfully cloned in 1953, by [[Theodore Puck]] and [[Philip I. Marcus]] at the [[University of Colorado, Denver]].{{cite journal|author1=Puck, T.T.|author2=Marcus, P.I.|year=1955|title=A rapid method for viable cell titration and clone production with Hela cells in tissue culture: The use of X-irradiated cells to supply conditioning factors|journal=Proceedings of the National Academy of Sciences of the United States of America|publication-date=July 15, 1955|volume=41|issue=7|pages=432–437|bibcode=1955PNAS...41..432P|doi=10.1073/pnas.41.7.432|pmc=528114|pmid=16589695|doi-access=free}} Since then, HeLa cells have "continually been used for research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits."{{cite web|title=Wonder Woman: The Life, Death, and Life After Death of Henrietta Lacks, Unwitting Heroine of Modern Medical Science|url=http://www.citypaper.com/news/story.asp?id=3426|last1=Smith|first1=Van|date=April 17, 2002|work=[[Baltimore City Paper]]|archive-url=https://web.archive.org/web/20040814160109/http://www.citypaper.com/news/story.asp?id=3426|archive-date=August 14, 2004|access-date=2 March 2017}} According to author [[Rebecca Skloot]], by 2009, "more than 60,000 scientific articles had been published about research done on HeLa [cells], and that number was increasing steadily at a rate of more than 300 papers each month."{{cite book|last=Skloot|first=Rebecca|title=The Immortal Life of Henrietta Lacks|title-link=The Immortal Life of Henrietta Lacks|publisher=Crown/Random House|year=2010|isbn=978-1-4000-5217-2|location=New York|author-link=Rebecca Skloot}} [36] => [37] => === Polio eradication === [38] => HeLa cells were used by [[Jonas Salk]] to test the first [[polio vaccine]] in the 1950s. They were observed to be easily infected by the [[poliomyelitis]] virus, causing infected cells to die. This made HeLa cells highly desirable for polio vaccine testing, since results could be easily obtained. A large volume of HeLa cells were needed for the testing of Salk's polio vaccine, prompting the [[National Foundation for Infantile Paralysis]] (NFIP) to find a facility capable of mass-producing HeLa cells.{{cite journal|last1=Masters|first1=John R.|year=2002|title=HeLa cells 50 years on: The good, the bad and the ugly|journal=Nature Reviews Cancer|volume=2|issue=4|pages=315–319|doi=10.1038/nrc775|pmid=12001993|s2cid=991019}} In the spring of 1953, a cell culture factory was established at [[Tuskegee University]] to supply Salk and other labs with HeLa cells.{{cite journal|last1=Turner|first1=Timothy|year=2012|title=Development of the Polio Vaccine: A Historical Perspective of Tuskegee University's Role in Mass Production and Distribution of HeLa Cells|journal=Journal of Health Care for the Poor and Underserved|volume=23|issue=4a|pages=5–10|doi=10.1353/hpu.2012.0151|pmc=4458465|pmid=23124495}} Less than a year later, Salk's vaccine was ready for human trials.{{cite journal|last1=Brownlee|first1=K. A.|year=1955|title=Statistics of the 1954 Polio Vaccine Trials*|journal=Journal of the American Statistical Association|volume=50|issue=272|pages=1005–1013|doi=10.1080/01621459.1955.10501286}} [39] => [40] => === Virology === [41] => HeLa cells have been used in testing how [[parvovirus]] infects cells of humans, dogs, and cats.{{cite journal|last=Parker|first=J|author2=Murphy W|author3=Wang D|author4=O'Brien S|author5=Parrish C|year=2001|title=Canine and feline parvoviruses can use human or feline transferrin receptors to bind, enter, and infect cells|journal=Journal of Virology|volume=75|issue=8|pages=3896–3902|doi=10.1128/JVI.75.8.3896-3902.2001|pmc=114880|pmid=11264378}} These cells have also been used to study viruses such as the [[oropouche virus]] (OROV). OROV causes disruption of cells in culture; the cells start to degenerate shortly after they are infected, causing [[viral induction of apoptosis]].{{cite journal|author1=Acrani, G.O.|author2=Gomes, R.|author3=Proença-Módena, J.L.|author4=da Silva, A.F.|author5=Carminati, P.O.|author6=Silva, M.L.|author7=Santos, R.I.|author8=Arruda, E.|year=2010|title=Apoptosis induced by Oropouche virus infection in HeLa cells is dependent on virus protein expression|journal=Virus Research|volume=149|issue=1|pages=56–63|doi=10.1016/j.virusres.2009.12.013|pmid=20080135|doi-access=free}} HeLa cells have been used to study expression of the [[Human papillomavirus|papillomavirus]] E2 and apoptosis.{{cite journal|author1=Hou, S.Y.|author2=Wu, S.|author3=Chiang, C.|year=2002|title=Transcriptional activity among high and low risk human papillomavirus E2 proteins correlates with E2 DNA binding|journal=The Journal of Biological Chemistry|volume=277|issue=47|pages=45619–45629|doi=10.1074/jbc.M206829200|pmid=12239214|s2cid=9203953|doi-access=free}} HeLa cells have also been used to study the ability of the [[canine distemper]] virus to induce [[apoptosis]] in cancer cell lines,{{cite journal|author1=del Puerto, H.L.|author2=Martins, A.S.|author3=Milsted, A.|author4=Souza-Fagundes, E.M.|author5=Braz, G.F.|author6=Hissa, B.|author7=Andrade, L.O.|author8=Alves, F.|author9=Rajão, D.S.|author10=Leite, R.C.|author11=Vasconcelos, A.C.|year=2011|title=Canine distemper virus induces apoptosis in cervical tumor derived cell lines|journal=Virol. J.|volume=8|issue=1|page=334|doi=10.1186/1743-422X-8-334|pmc=3141686|pmid=21718481 |doi-access=free }} which could play an important role in developing treatments for tumor cells resistant to radiation and chemotherapy. [42] => [43] => HeLa cells have also been instrumental in the development of [[Human papillomavirus infection|human papilloma virus]] (HPV) vaccines. In the 1980s, [[Harald zur Hausen]] found that Lacks's cells from the original biopsy contained HPV-18, which was later found to be the cause of the aggressive cancer that had killed her. His work in linking HPV with cervical cancer won him a Nobel Prize and led to the development of HPV vaccines, which are predicted to reduce the number of deaths from cervical cancer by 70%.{{Cite web|title=5 Contributions HeLa Cells Have Made to Science|url=https://www.technologynetworks.com/cell-science/lists/5-contributions-hela-cells-have-made-to-science-305036|website=Cell Science from Technology Networks|language=en|access-date=2020-03-25 |last=MacDonald |first=Anna |date=13 June 2018}} [44] => [45] => Over the years, HeLa cells have been infected with various types of viruses, including HIV, Zika, mumps, and herpesviruses to test and develop new vaccines and drugs. Dr. Richard Axel discovered that the addition of the CD4 protein to HeLa cells enabled them to be infected with HIV, allowing the virus to be studied.{{Cite journal|last1=Mondor|first1=Isabelle|last2=Ugolini|first2=Sophie|last3=Sattentau|first3=Quentin J.|date=1998-05-01|title=Human Immunodeficiency Virus Type 1 Attachment to HeLa CD4 Cells Is CD4 Independent and gp120 Dependent and Requires Cell Surface Heparans|journal=Journal of Virology|volume=72|issue=5|pages=3623–3634|doi=10.1128/jvi.72.5.3623-3634.1998|pmid=9557643|pmc=109583|issn=1098-5514}} In 1979, scientists learned that the measles virus constantly mutates when it infects HeLa cells,{{Cite journal|last1=Wechsler|first1=Steven L.|last2=Rustigian|first2=Robert|last3=Stallcup|first3=Kathryn C.|last4=Byers|first4=Karen B.|last5=Winston|first5=Stuart H.|last6=Fields|first6=Bernard N.|date=1979|title=Measles Virus-Specified Polypeptide Synthesis in Two Persistently Infected HeLa Cell Lines|journal=Journal of Virology|volume=31|issue=3|pages=677–684|doi=10.1128/jvi.31.3.677-684.1979|pmid=513191|pmc=353496|issn=0022-538X}} and in 2019 they found that Zika cannot multiply in HeLa cells.{{Cite journal|last1=Li|first1=Li|last2=Collins|first2=Natalie D.|last3=Widen|first3=Steven G.|last4=Davis|first4=Emily H.|last5=Kaiser|first5=Jaclyn A.|last6=White|first6=Mellodee M.|last7=Greenberg|first7=M. Banks|last8=Barrett|first8=Alan D. T.|last9=Bourne|first9=Nigel|last10=Sarathy|first10=Vanessa V.|date=2019-08-20|title=Attenuation of Zika Virus by Passage in Human HeLa Cells|journal=Vaccines|volume=7|issue=3|pages=93|doi=10.3390/vaccines7030093|issn=2076-393X|pmc=6789458|pmid=31434319|doi-access=free}} [46] => [47] => === Cancer === [48] => HeLa cells have been used in a number of cancer studies, including those involving sex steroid hormones, such as [[estradiol]] and [[estrogen]], and [[estrogen receptor]]s, along with estrogen-like compounds, such as [[quercetin]], which has cancer-reducing properties.{{cite journal|author1=Pamela Bulzomi|author2=Paola Galluzzo|author3=Alessandro Bolli|author4=Stefano Leone|author5=Filippo Acconcia|author6=Maria Marino|year=2012|title=The pro-apoptotic effect of quercetin in cancer cell lines requires ERβ-dependent signals|journal=Journal of Cellular Physiology|volume=227|issue=5|pages=1891–1898|doi=10.1002/jcp.22917|pmid=21732360|s2cid=24034074}} There have also been studies on HeLa cells, involving the effects of flavonoids and antioxidants with estradiol on cancer cell proliferation. [49] => [50] => In 2011, HeLa cells were used in tests of novel [[heptamethine dyes]] IR-808 and other analogues, which are currently being explored for their unique uses in medical diagnostics, the individualized treatment of cancer patients with the aid of [[Photodynamic therapy|PDT]], co-administration with other drugs, and [[irradiation]].{{cite journal|vauthors=Tan X, Luo S, Wang D, Su Y, Cheng T, Shi C|year=2011|title=A NIR heptamethine Dye with intrinsic cancer targeting, imaging and photosynthesizing properties|journal=Journal of Biomaterials China|volume=33|issue=7|pages=2230–2239|doi=10.1016/j.biomaterials.2011.11.081|pmid=22182749}}{{cite journal|author1=Pene, F.|author2=Courtine, E.|author3=Cariou, A.|author4=Mira, J.P.|year=2009|title=Toward theranostics|journal=Crit Care Med|volume=37|issue=1 Suppl|pages=S50–S58|doi=10.1097/CCM.0b013e3181921349|pmid=19104225|s2cid=37043095}} HeLa cells have been used in research involving [[fullerenes]] to induce apoptosis as a part of [[photodynamic therapy]], as well as in ''in vitro'' cancer research using cell lines.{{cite journal|last=Briiuner.|first=Thomas|author2=Dieter F. Hulser|year=1990|title=Tumor Cell Invasion and Gap Junctional Communication|url=http://elib.uni-stuttgart.de/opus/volltexte/2011/6882/pdf/huel63.pdf|journal=Invasion Metastasis|volume=10|pages=31–34|access-date=3 April 2012}} HeLa cells have also been used to define cancer markers in RNA, and have been used to establish an [[RNAi-Based Identification System and interference of Specific Cancer Cells|RNAi Based Identification System and Interference of Specific Cancer Cells]].{{cite journal|author1=Xie, Z.|author2=Wroblewska, L.|author3=Prochazka, L.|author4=Weiss, R.|author5=Benenson, Y.|year=2011|title=Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells|url=http://courses.cs.washington.edu/courses/cse599x/10sp/RNAi_circuits.pdf|journal=Science|volume=333|issue=6047|pages=1307–1311|bibcode=2011Sci...333.1307X|doi=10.1126/science.1205527|pmid=21885784|s2cid=13743291}} [51] => [52] => In 2014, HeLa cells were shown to provide a viable cell line for tumor [[xenografts]] in [[C57BL/6]] nude mice,{{cite journal|last1=Arjomandnejad|first1=M|display-authors=etal|date=2014|title=HeLa cell line xenograft tumor as a suitable cervical cancer model: growth kinetic characterization and immunohistochemistry array|url=https://pdfs.semanticscholar.org/4584/d1d5efb89d7ab1001a96ba5f7d2a69596cfe.pdf|archive-url=https://web.archive.org/web/20180425115027/https://pdfs.semanticscholar.org/4584/d1d5efb89d7ab1001a96ba5f7d2a69596cfe.pdf|url-status=dead|archive-date=2018-04-25|journal=Archives of Iranian Medicine|volume=17|issue=4|pages=273–277|pmid=24724604|s2cid=25652255}} and were subsequently used to examine the ''in vivo'' effects of [[fluoxetine]] and [[cisplatin]] on cervical cancer. [53] => [54] => === Genetics === [55] => In 1953, a lab mistake involving mixing HeLa cells with the wrong liquid allowed researchers for the first time to see and count each chromosome clearly in the HeLa cells with which they were working. This accidental discovery led scientists [[Joe Hin Tjio]] and [[Albert Levan]] to develop better techniques for staining and counting chromosomes. They were the first to show that humans have 23 pairs of chromosomes rather than 24, as was previously believed. This was important for the study of developmental disorders, such as [[Down syndrome]], that involve the number of chromosomes. [56] => [57] => In 1965, [[Henry Harris (scientist)|Henry Harris]] and John Watkins created the first human-animal hybrid by fusing HeLa cells with mouse embryo cells. This enabled advances in mapping genes to specific chromosomes, which would eventually lead to the [[Human Genome Project]]. [58] => [59] => === Space microbiology === [60] => In the 1960s, HeLa cells were sent on the Soviet satellite [[Korabl-Sputnik_3|Sputnik-6]] and human space missions to determine the long term effects of space travel on living cells and tissues. Scientists discovered that HeLa cells divide more quickly in zero gravity.{{Cite web|title=Significant Research Advances Enabled by HeLa Cells|url=https://osp.od.nih.gov/scientific-sharing/hela-cells-timeline/|last=Davies|first=Preston|website=Office of Science Policy|language=en-US|access-date=2020-03-25|archive-date=February 2, 2020|archive-url=https://web.archive.org/web/20200202220227/https://osp.od.nih.gov/scientific-sharing/hela-cells-timeline/|url-status=dead}} [61] => [62] => == Analysis == [63] => [64] => ===Telomerase=== [65] => The HeLa [[cell line]] was derived for use in [[cancer research]]. These cells proliferate abnormally rapidly, even compared with other cancer cells. Like many other cancer cells,[https://www.nobelprize.org/nobel_prizes/medicine/laureates/2009/press.html The Nobel Prize in Physiology or Medicine 2009] on nobelprize.org HeLa cells have an active version of [[telomerase]] during cell division,{{cite journal |vauthors=Ivanković M, Cukusić A, Gotić I, Skrobot N, Matijasić M, Polancec D, Rubelj I | title = Telomerase activity in HeLa cervical carcinoma cell line proliferation | journal = Biogerontology | volume = 8 | issue = 2 | pages = 163–72 | year = 2007 | pmid = 16955216 | doi = 10.1007/s10522-006-9043-9 | s2cid = 9390790 }} which copies telomeres over and over again. This prevents the incremental shortening of [[telomere]]s that is implicated in aging and eventual cell death. In this way, the cells circumvent the [[Hayflick limit]], which is the limited number of cell divisions that most normal cells can undergo before becoming [[Senescence|senescent]]. This results in unlimited cell division and immortality. [66] => [67] => ===Chromosome number=== [68] => [[Horizontal gene transfer]] from [[human papillomavirus]] 18 (HPV18) to [[human]] cervical cells created the HeLa genome, which is different from Henrietta Lacks's genome in various ways, including the number of chromosomes. HeLa cells are rapidly dividing cancer cells, and the number of chromosomes varies during cancer formation and cell culture. The current estimate (excluding very tiny fragments) is a "hypertriploid chromosome number (3n+)", which means 76 to 80 total chromosomes (rather than the normal diploid number of 46) with 22–25 clonally abnormal chromosomes, known as "HeLa signature chromosomes".{{cite journal|vauthors=Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T|year=1999|title=Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping|journal=Cancer Res.|volume=59|issue=1|pages=141–50|pmid=9892199}}{{cite journal|vauthors=Landry JJ, Pyl PT, Rausch T, Zichner T, Tekkedil MM, Stütz AM, Jauch A, Aiyar RS, Pau G, Delhomme N, Gagneur J, Korbel JO, Huber W, Steinmetz LM|year=2013|title=The genomic and transcriptomic landscape of a HeLa cell line|journal=G3: Genes, Genomes, Genetics|volume=3|issue=8|pages=1213–24|doi=10.1534/g3.113.005777|pmc=3737162|pmid=23550136}}{{cite journal|author1=Bottomley, R.H.|author2=Trainer, A.L.|author3=Griffin, M.J.|year=1969|title=Enzymatic and chromosomal characterization of HeLa variants|journal=J. Cell Biol.|volume=41|issue=3|pages=806–15|doi=10.1083/jcb.41.3.806|pmc=2107821|pmid=5768876}}{{cite journal|author1=Andrew Adey|author2=Joshua N. Burton|author3=Jacob O. Kitzman|author4=Joseph B. Hiatt|author5=Alexandra P. Lewis|author6=Beth K. Martin|author7=Ruolan Qiu|author8=Choli Lee|author9=Jay Shendure|year=2013|title=The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line|journal=Nature|publication-date=August 8, 2013|volume=500|issue=7461|pages=207–211|bibcode=2013Natur.500..207A|doi=10.1038/nature12064|pmc=3740412|pmid=23925245}} The signature chromosomes can be derived from multiple original chromosomes, making summary counts based on original numbering challenging. Researchers have also noted how stable these aberrant [[karyotype]]s can be. Studies that combined spectral karyotyping, [[Fluorescent in situ hybridization|FISH]], and conventional cytogenic techniques have shown that the detected chromosomal aberrations may be representative of advanced cervical carcinomas and were probably present in the primary tumor, since the HeLa genome has remained stable, even after years of continued cultivation. [69] => [70] => ===Complete genome sequence=== [71] => The complete [[genome]] of HeLa cells was [[sequencing|sequenced]] and published on 11 March 2013,{{cite journal | journal=G3: Genes, Genomes, Genetics |year=2013 |volume=3|issue=8 |pages=1213–24 | doi= 10.1534/g3.113.005777 | title=The genomic and transcriptomic landscape of a HeLa cell line |vauthors= Landry JJ, Pyl PT, Rausch T, Zichner T, Tekkedil MM, Stütz AM, Jauch A, Aiyar RS, Pau G, Delhomme N, Gagneur J, Korbel JO, Huber W, Steinmetz LM | pmid=23550136 | pmc=3737162 }}{{Cite journal|url=http://www.nature.com/news/most-popular-human-cell-in-science-gets-sequenced-1.12609 |title=Most popular human cell in science gets sequenced |journal=Nature |author=Callaway, Ewen |doi=10.1038/nature.2013.12609 |date=March 15, 2013 |s2cid=87549985 |access-date=8 August 2013}} without the Lacks family's knowledge.{{Cite journal|url=http://www.nature.com/news/hela-publication-brews-bioethical-storm-1.12689 |title=HeLa publication brews bioethical storm |journal=Nature |author=Callaway, Ewen |doi=10.1038/nature.2013.12689 |date=March 27, 2013 |s2cid=88020977 |access-date=8 August 2013|doi-access=free }} Concerns were raised by the family, so the authors voluntarily withheld access to the sequence data. [[Jay Shendure]] led a HeLa sequencing project at the University of Washington, which resulted in a paper that had been accepted for publication in March 2013 – but that was also put on hold while the Lacks family's privacy concerns were addressed.{{Cite journal|title=Deal done over HeLa cell line |journal=Nature |volume=500 |issue=7461 |pages=132–133 |author=Callaway, Ewen |doi=10.1038/500132a |pmid=23925220 |date=August 7, 2013 |bibcode=2013Natur.500..132C|doi-access=free }} On 7 August 2013, [[NIH]] director [[Francis Collins]] announced a policy of controlled access to the cell line genome, based on an agreement reached after three meetings with the Lacks family.{{cite web |url=http://nih.gov/news/health/aug2013/nih-07.htm |title=NIH, Lacks family reach understanding to share genomic data of HeLa cells |publisher=The National Institutes of Health |date=August 7, 2013 |access-date=8 August 2013 |archive-date=August 11, 2013 |archive-url=https://web.archive.org/web/20130811082728/http://www.nih.gov/news/health/aug2013/nih-07.htm |url-status=dead }} A data-access committee will review requests from researchers for access to the genome sequence, under the criteria that the study is for medical research and that the users will abide by terms in the HeLa Genome Data Use Agreement, which includes that all NIH-funded researchers will deposit the data in a single database for future sharing. The committee consists of six members, including representatives from the medical, scientific, and bioethics fields, as well as two members of the Lacks family. In an interview, Collins praised the Lacks family's willingness to participate in a situation that was thrust upon them. He described the whole experience with them as "powerful," saying that it brought together "science, scientific history and ethical concerns" in a unique way.{{cite journal |doi=10.1038/nature.2013.13521 |date=August 7, 2013 |title=NIH director explains HeLa agreement |last1=Callaway |first1=Ewen |journal=Nature|s2cid=190871214 }} [72] => [73] => ==Contamination== [74] => HeLa cells are sometimes difficult to control, because they adapt to growth in tissue culture plates and invade and outcompete other cell lines. Through improper maintenance, they have been known to contaminate other cell cultures in the same laboratory, interfering with biological research and forcing researchers to declare many results invalid. The degree of HeLa cell contamination among other cell types is unknown, because few researchers test the identity or purity of already established cell lines. It has been shown that a substantial fraction of ''[[in vitro]]'' cell lines are contaminated with HeLa cells; estimates range from 10% to 20%. This observation suggests that any cell line may be susceptible to a degree of contamination. [[Stanley Gartler]] (1967) and [[Walter Nelson-Rees]] (1975) were the first to publish on contamination of various cell lines by HeLa cells. Gartler noted that "with the continued expansion of cell culture technology, it is almost certain that both interspecific and intraspecific contamination will occur." [75] => [76] => HeLa cell contamination has become a pervasive worldwide problem – affecting even the laboratories of many notable physicians, scientists, and researchers, including [[Jonas Salk]]. The HeLa contamination problem also contributed to [[Cold War]] tensions. The USSR and the USA had begun to cooperate in the [[war on cancer]] launched by President [[Richard Nixon]], only to find that the exchanged cells were contaminated by HeLa.{{Cite book|last=Gold, Michael |title=A conspiracy of cells: one woman's immortal legacy and the medical scandal it caused|date=1986|publisher=State University of New York Press|isbn=0-88706-099-4|oclc=12805138}} [77] => [78] => Rather than focus on how to resolve the problem of HeLa cell contamination, many scientists and science writers continue to document this problem as simply a contamination issue – caused not by human error or shortcomings but by the hardiness, proliferation, or overpowering nature of HeLa cells.{{cite journal|vauthors=Wang H, Huang S, Shou J, Su EW, Onyia JE, Liao B, Li S|year=2006|title=Comparative analysis and integrative classification of NCI60 cell lines and primary tumors using gene expression profiling data|journal=BMC Genomics|volume=7|issue=1|page=166|doi=10.1186/1471-2164-7-166|pmc=1525183|pmid=16817967 |doi-access=free }} Recent data suggest that cross-contamination is still a major problem with modern cell cultures.{{cite journal|author=Nardone, R.M.|year=2007|title=Eradication of cross-contaminated cell lines: A call for action|url=http://www.sivb.org/publicPolicy_Eradication.pdf|url-status=dead|journal=Cell Biology and Toxicology|volume=23|issue=6|pages=367–372|citeseerx=10.1.1.432.8581|doi=10.1007/s10565-007-9019-9|pmid=17522957|s2cid=21077969|archive-url=https://wayback.archive-it.org/all/20081217011516/http://www.sivb.org/publicPolicy_Eradication.pdf|archive-date=2008-12-17|access-date=2007-12-04}} The International Cell Line Authentication Committee (ICLAC) notes that many cases of cell line misidentification are the result of cross-contamination of the culture by another, faster-growing cell line. This calls into question the validity of the research done using contaminated cell lines, as certain attributes of the contaminant, which may come from an entirely different species or tissue, may be misattributed to the cell line under investigation.{{cite web|title=ATCC® Standards Development Organization: The International Cell Line Authentication Committee (ICLAC)|url=http://standards.atcc.org/kwspub/home/the_international_cell_line_authentication_committee-iclac_/|publisher=Standards.atcc.org|access-date=2013-06-27|archive-date=May 8, 2013|archive-url=https://web.archive.org/web/20130508075954/http://standards.atcc.org/kwspub/home/the_international_cell_line_authentication_committee-iclac_/|url-status=dead}} [79] => [80] => ==New species proposal== [81] => HeLa cells were described by [[evolutionary biologist]] [[Leigh Van Valen]] as an example of the contemporary creation of a new species, dubbed ''Helacyton gartleri'', owing to their ability to replicate indefinitely and their non-human number of [[chromosomes]]. The species was named after geneticist [[Stanley M. Gartler]], whom Van Valen credits with discovering "the remarkable success of this species".{{cite journal |vauthors=Maiorana VC, Van Valen LM |title=Hela, a new microbial species |journal=Evolutionary Theory |date=7 February 1991 |language=en |url=http://www.mn.uio.no/cees/english/services/evolutionary-theory/volume-10/vol-10-no-2-pages-71-74-l-van-valen-and-v-c-mairorana-hela-a-new-microbial-species.pdf |url-status=live |archive-url=https://web.archive.org/web/20180329120825/http://www.mn.uio.no/cees/english/services/evolutionary-theory/volume-10/vol-10-no-2-pages-71-74-l-van-valen-and-v-c-mairorana-hela-a-new-microbial-species.pdf |archive-date=29 March 2018 |volume=10|issue=2|pages=71–4}} His argument for speciation depends on these points: [82] => *the chromosomal incompatibility of HeLa cells with human cells; [83] => *the [[ecological niche]] of HeLa cells; [84] => *their ability to persist and expand well beyond the desires of human cultivators; [85] => *the possession by HeLa cells of their own clonal [[karyotype]], defining it as a distinct species.{{cite journal |pmid=21666415 |year=2011 |last1=Duesberg |first1=P |last2=Mandrioli |first2=D |last3=McCormack |first3=A |last4=Nicholson |first4=JM |title=Is carcinogenesis a form of speciation? |volume=10 |issue=13 |pages=2100–2114 |journal=Cell Cycle |place=Georgetown, Texas |doi=10.4161/cc.10.13.16352|doi-access=free }} [86] => [87] => Van Valen proposed the new family Helacytidae and the genus ''Helacyton'', and in the same paper proposed a new species for HeLa cells.{{cite journal |title=HeLa, a new microbial species |journal=Evolutionary Theory & Review |volume=10 |issn=1528-2619 |pages=71–74 |year=1991 |last1=van Valen |first1=L.M. |last2=Maiorana |first2=V.C. |author-link1=Leigh Van Valen }} [88] => [89] => However, this proposal was not taken seriously by other prominent evolutionary biologists, nor by scientists in other disciplines. Van Valen's argument that HeLa are a new species does not fulfill the criteria for an independent unicellular asexually reproducing species, because of the notorious instability of HeLa's karyotype and their lack of a strict ancestral-descendant lineage.{{cite journal |author=Cohan, FM |title= What are bacterial species? |journal= Annu. Rev. Microbiol. |volume=56 |pages=457–487 |year=2002 |doi=10.1146/annurev.micro.56.012302.160634 |pmid=12142474 |issue=1|url= http://wesscholar.wesleyan.edu/cgi/viewcontent.cgi?article=1366&context=div3facpubs }} [90] => [91] => ==Gallery== [92] => [93] => File:HeLa-II.jpg|Multiphoton fluorescence image of HeLa cells stained with the actin- binding toxin phalloidin (red), microtubules (cyan), and cell nuclei (blue). Nikon RTS2000MP custom laser scanning microscope. [94] => File:HeLa-III.jpg|Multiphoton fluorescence image of HeLa cells with cytoskeletal microtubules (magenta) and DNA (cyan). Nikon RTS2000MP custom laser scanning microscope. [95] => File:HeLa-V.jpg|Scanning electron micrograph of just-divided HeLa cells. Zeiss Merlin HR-SEM. [96] => File:HeLa cells stained with Hoechst 33258.jpg|HeLa cells stained with [[Hoechst stain|Hoechst 33258]] [97] => File:Ki67-Tubulin-2.jpg|HeLa cells grown in culture and stained with antibody to [[tubulin]] (green), antibody to [[Ki-67 (protein)|Ki-67]] (red), and the blue DNA binding dye DAPI. The tubulin antibody shows the distribution of [[microtubules]] and the Ki-67 antibody is expressed in cells about to divide. Preparation, antibodies and image courtesy of [[EnCor Biotechnology]]. [98] => File:Nuclear envelope of one cancerous HeLa cell.png|A volumetric surface render (red) of the [[nuclear envelope]] of one HeLa cell. The cell was observed in 300 slices on [[electron microscopy]] and the nuclear envelope was automatically segmented and rendered. One vertical and one horizontal slice are added for reference. [99] => File:Plasma Membrane and Nuclear Envelope.gif|Plasma membrane and nuclear envelope of one Hela cell, displayed as a volumetric surface rendering. Left and centre: the plasma membrane in blue, with transparency, and the nuclear envelope in solid cyan. Right: the plasma membrane without transparency and the same angle of view as the centre picture. The membranes have been segmented from data acquired by electron microscopy. [100] => [101] => [102] => ==In media== [103] => [104] => *The 1997 documentary ''[[The Way of All Flesh (documentary)|The Way of All Flesh]]'' by [[Adam Curtis]] explained the history of HeLa cells and their implications for medicine and society.{{cite journal |url=http://genome.ch.bbc.co.uk/efaa1adefb274189a158fc5676627d32 |title=Modern Times: The Way of All Flesh - BBC Two England - 19 March 1997 |issue=3815 |pages=92 |journal=The Radio Times |date=1997-03-13 }} [105] => [106] => *A 2010 episode of [[Law & Order]], "Immortal", was heavily based on the story of Henrietta Lacks and the HeLa cell line, using the fictional "NaRo" cells as a stand-in.{{Cite web|last=Thomas|first=June|date=2010-05-19|title=Ripped From Which Headline? "Immortal"|url=https://slate.com/culture/2010/05/ripped-from-which-headline-immortal.html|access-date=2021-08-03|website=Slate Magazine|language=en}} [107] => [108] => *The story of how the HeLa cell line came to be was also the subject of a 2010 episode of the podcast [[Radiolab]].{{cite web | url=https://www.wnycstudios.org/podcasts/radiolab/segments/91716-henriettas-tumor| title=Henrietta's Tumor }} [109] => [110] => *HeLa cells were the subject of a 2010 book by [[Rebecca Skloot]], ''[[The Immortal Life of Henrietta Lacks]]'', investigating the historical context of the cell line and how the Lacks family was involved in its use. [111] => *A 2017 HBO film, ''[[The Immortal Life of Henrietta Lacks (film)|The Immortal Life of Henrietta Lacks]]'', was based on the book. The film starred [[Oprah Winfrey]], [[Sylvia Grace Crim]], and [[Rocky Carroll]], with [[Renee Elise Goldsberry]] as Henrietta Lacks. Author Rebecca Skloot also appeared as a character in the film, portrayed by [[Rose Byrne]].{{cite web|url=http://rebeccaskloot.com/the-immortal-life/|title=About The Immortal Life of Henrietta Lacks|publisher=Rebecca Skloot|access-date=24 April 2018}} [112] => [113] => == See also == [114] => *[[Clonally transmissible cancer]] [115] => *''[[Moore v. Regents of the University of California]]'', case that set precedent for discarded tissue [116] => *[[List of contaminated cell lines]] [117] => *[[WI-38]] [118] => [119] => == References == [120] => {{reflist|colwidth=35em}} [121] => [122] => ==Further reading== [123] => *{{cite book |author=Hannah Landecker |chapter=Immortality, In Vitro: A History of the HeLa Cell Line |editor=Brodwin, Paul |title=Biotechnology and culture: bodies, anxieties, ethics |publisher=[[Indiana University Press]] |location=Bloomington |year=2000 |pages=[https://archive.org/details/biotechnologycul0000unse/page/53 53–74] |isbn=978-0-253-21428-7 |author-link=Hannah Landecker |chapter-url=https://archive.org/details/biotechnologycul0000unse/page/53 }} [124] => *{{cite book |author=Rebecca Skloot |year=2010|title=The Immortal Life of Henrietta Lacks|title-link=The Immortal Life of Henrietta Lacks|author-link=Rebecca Skloot}} [125] => [126] => == External links == [127] => {{commons category|HeLa cells}} [128] => * [http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CCL-2&Template=cellBiology HeLa (CCL-2 Cells)] in the [[American Type Culture Collection|ATCC]] database [129] => * {{MeshName|HeLa+Cells|3=HeLa Cells}} [130] => *[http://cell-lines.toku-e.com/Cell-Lines_1434.html HeLa Transfection and Selection Data for HeLa Cells] [131] => *[[Rebecca Skloot]], [http://rebeccaskloot.com/the-immortal-life/ The Immortal Life of Henrietta Lacks] book website with additional features (photo/video/audio) [132] => *[http://www.henriettalacksfoundation.org The Henrietta Lacks Foundation], a foundation established to, among other things, help provide scholarship funds and health insurance to Henrietta Lacks's family. [133] => *[https://web.archive.org/web/20040814160109/http://www.citypaper.com/news/story.asp?id=3426 "Wonder Woman: The Life, Death, and Life After Death of Henrietta Lacks, Unwitting Heroine of Modern Medical Science"] by Van Smith [134] => *[http://www.lrb.co.uk/v22/n08/enri01_.html "What's Left of Henrietta Lacks?"] by Anne Enright [135] => *[http://ccdb.ucsd.edu/sand/main?stype=lite&keyword=hela&Submit=Go&event=display&start=1 Cell Centered Database – HeLa cell] [136] => *[https://www.cellosaurus.org/CVCL_0030 Cellosaurus entry for HeLa] [137] => *[https://www.hopkinsmedicine.org/henriettalacks/index.html The Legacy of Henrietta Lacks] [138] => [139] => {{Self-replicating organic structures}} [140] => {{Organisms et al.}} [141] => [142] => [[Category:Human cell lines]] [143] => [[Category:Bioethics]] [144] => [[Category:Johns Hopkins Hospital]] [145] => [[Category:Cellular senescence]] [146] => [[Category:1951 in biotechnology]] [147] => [[Category:Cervical cancer]] [] => )
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HeLa

HeLa cells are a line of immortal human cells that have been widely used in scientific research since their discovery in 1951. Named after Henrietta Lacks, the African-American woman from whom the cells were taken without her knowledge or consent, HeLa cells have played a critical role in various fields such as vaccine development, cancer research, and gene mapping.

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Named after Henrietta Lacks, the African-American woman from whom the cells were taken without her knowledge or consent, HeLa cells have played a critical role in various fields such as vaccine development, cancer research, and gene mapping. These cells are unique in their ability to divide indefinitely, giving researchers an unlimited supply for experimentation. However, their use has raised ethical concerns regarding informed consent and privacy rights. Despite these controversies, HeLa cells have revolutionized medicine and continue to contribute to groundbreaking discoveries in science.

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